FTIR Microspectroscopy

FTIR Microspectroscopy (INVENIO FTIR Spectrometer equipped with HYPERION 3000 Vis-IR Microscope, Bruker Optics)

FTIR Microspectroscopy is a powerful and label-free vibrational technique, used to study the chemical composition of biological samples (such as tissues, biological fluids, and cells) and organic and inorganic compounds (including polymers and biocomposites), with a spatial resolution up to ~2.56 mm. FTIR spectroscopy is sensitive to polar (usually antisymmetric) group vibrations. The coupling with the optical microscope allows to correlate the morphological features of biological non-homogeneous samples (such as tissues and cells) with the chemical composition of the sample itself.
Hyperspectral characterization of the MSTO-211H cell spheroid model: A FPA–FTIR imaging approach			10.1016/j.clispe.2021.100011
The Invenio FTIR spectrometer is coupled with a Hyperion 3000 Vis-IR microscope. It is equipped with a HgCdTe (MCT_A) detector for single point analysis (ca- 20 micron spatial resolution) and a bidimensional Focal Plane Array (FPA) detector for imaging analysis (64×64 pixel/spectra, covering an area of ~164×164 μm2, with a spatial resolution of 2.56 micron). The acquisition of spectra can be performed in reflection or transmission mode in the MIR (Medium InfraRed) region (4000–800 cm−1).

FPA-FTIR analysis of triple-negative breast cancer samples
The instrument is also implemented with the Platinum ATR, a single reflection diamond ATR (Attenuated Total Reflectance) accessory, equipped with a DTGS detector, and which can be mounted in the sample compartment of the Invenio interferometer. ATR-FTIR spectroscopy is an in situ technique applied to investigate molecular compounds and biological samples in aqueous systems, enhancing the achievable spatial resolution.